BACTERIOLOGICAL STUDIES OF CLOSTRIDIUM PERFRINGENS IN FISH

Document Type : Original Article

Authors

1 Central Lab., Vet. Hospital, Faculty of Vet. Med., Zagazig Univ., Egypt

2 Animal Health Research Institute, Zagazig, Egypt

Abstract

The current research was carried out on one hundred and thirteen random fish samples (56 processed and 57 unprocessed) collected from different localities in El-Sharkia Governorate, Egypt to obtain a complete picture of C. perfringens. Bacteriological and biochemical examination was done on the isolates. Nagler's test was applied on the recovered. C. perfringens   isolates. The total incidence of positive samples from 56 processed fish samples were 32 isolates (57.1%) and from 57 unprocessed fish samples were 34 isolates (59.6%). The. Typing of C. perfringens isolates revealed that the incidence of toxigenic and non-toxigenic isolates were 84.8 and 15.2% respectively. Typing of toxigenic strains of C. perfringens revealed that C. perfringens type A was the most predominant one comparing with type D. Immune diffusion test showed that 17 and 11 toxins of types A and D gave identified reaction with incidences of 47.2 and 55% respectively.

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