DETECTION OF SALMONELLOSIS AND PASTEURELLOSIS IN DUCKS USING POLYMERASE CHAIN REACTION TECHNIQUE (PCR)

Ammar, Ahmed M.; Helmy, Salwa M.; Abd El – Wanis, Soad A.; Nabil, Nehal M.

doi:10.21608/kvmj.2012.112017

DETECTION OF SALMONELLOSIS AND PASTEURELLOSIS IN DUCKS USING POLYMERASE CHAIN REACTION TECHNIQUE (PCR)

Document Type : Original Article

Authors

¹ Bacteriology, Mycology and Immunology Dep., Fac. of Vet. Med., Zagzig Univ., Egypt.

² Bacteriology, Mycology and Immunology Dep., Fac. of Vet. Med., Kafrelsheikh Univ., Egypt.

³ Animal Health Research Institute, Dokki Lab., Giza, Egypt.

⁴ Animal Health Research Institute, Dakahlia branch, Egypt.

10.21608/kvmj.2012.112017

Abstract

A total of 198 samples from internal organs and fecal matter of diseased and healthy ducks breeds were collected from farms and backyards in Dakahlia governorate (Egypt). The samples were examined bacteriologically and by PCR. Sensitivity, PCR and pathogencity tests were applied. Fifteen samples (7.58%) were found to be positive for Salmonellosis and four strains were detected serologically (S. Infantis, S.Enteritidis, S. Inganda and S. Larochelle) also Untyped Salmonella strain was detected. Six samples (3.09 %) were found to be positive for Pasteurella multocida by bacteriological and PCR examinations.

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