ELECTROPHORETIC AND IMMUNOLOGICAL CHARAC-TERIZATION OF SPECIFIC ANTIGENS INDUCING IMMUNE RESISTANCE DERIVED FROM CATTLE TICK BOOPHILUS ANNULATUS

Document Type : Original Article

Authors

1 Biotechnology Dept., Animal Health Research Institute Dokki.

2 Port-Said Lab, Animal Health Research Institute

Abstract

SDS- PAGE analysis was carried out on salivary gland antigen (SGA) of the cattle tick Boophilus annulatus . A protein profile was observed with various polypeptide bands. On the other hand, has been determined the presence of the specific antigen on salivary gland extract by means of immunoblotting analysis using hyperimmune serum of rabbit inoculated with extract. Higher reactivity was observed at three polypeptides ( 48,42 and 32 KD).

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