THE EFFECT OF SERUM STORAGE FACTORS INFLUENCING THE REACTIVITY OF ANTIBODIES IN SERODIAGNOSTIC TESTS

Document Type : Original Article

Authors

1 Department of Microbiology Faculty of Veterinary Medicine, Kafr El-Sheikh Tanta University

2 Department of Animal Medicine Faculty of Veterinary Medicine, Kafr El-Sheikh Tanta University

Abstract

To investigatetheeffectofdifferentserumstoragemethodsonreactivity of bovineBrucellaantibodiesinserodiagnostictests,sera were collected fromBrucellainfected cattleandstoredeitherin a refrigerator at 4 ◦Cor in a deep freezer at   - 20 ◦C mean while reference serum samples were stored at 4 ◦C, - 20 ◦C and - 80 ◦C.
Monitoring of the Brucella antibody activity in stored samples, was performed by Slow Agglutination Test (SAT) on monthly basis for sixmonths and results obtained were analyzed in three dimensions. First, betweendifferenttimeintervals,second,betweendifferentstoragetemp-eratures and finally between collected sera and reference sera samples.
AnalysisoftheresultsrevealedasignificantreductionintiterofBrucella antibodies in sera stored at- 20 ◦C after two months of storage,whereas no significant difference could be detected in antibodies titer for up to four months of serum storage at 4 ◦C, meanwhile reference samples showed stability in titer of Brucella antibodies when compared with collected serum under the same storage temperatures.It was concluded thattheeffectof storagetemperature is more or less affected by quality of serum.
Since high quality serum is the cornerstone in sero-epidemiological studies and diagnosis, we recommended the necessity of sterilization of serum by filtration. Additionally, the constancy of cooling tempera-tureandtheavoidance of refreezing and thawing during storage should be considered in order to get accurate results from serum samples.

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Kafrelsheikh Veterinary Medical Journal
Volume 2, Issue 2
October 2004
Pages 29-42

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