EFFICACY OF BABESIA BIGEMINA EXOANTIGEN VACCINE FOR PROTECTION AGAINST BABESIA BIGEMINA INFECTION

Document Type : Original Article

Author

Lecturer of infectious diseases, Department of Animal Medicine, Faculty of Veterinary Medicine, Alexandria University.

Abstract

30 fattening calves1 year old were divided into 3 groups (10 in each), 1st group injected with B. bigemina exoantigen vaccine (240 mg of total protein combined with 2 mg saponin/dose) 2 doses with one month interval then Inoculated with 1×109 of Babesia bigemina infected RBCs after 90 days of 2nd dose of vaccine, 2nd group injected with B. bigemina exoantigen vaccine 2 doses with one month interval, while 3rd group injected with 2 mg saponin 2 doses with one month interval then Inoculated with 1×109 of Babesia bigemina infected RBCs after 90 days of 2nd dose of vaccine. Significant increase in antibody titers against B. bigemina at day 21 after 1st and 2nd dose of vaccination while no antibodies were detected in the non vaccinated groupas measured by ELISA. Lymphocyte transformation index  at day 15 after 1st and 2nd dose of vaccination was higher in vaccinated than non vaccinated animals. Immunized cattle with B. bigemina exoantigen vaccine were protected against virulent homologous challenge with 109 virulent b. bigemina infected RBCs and elicited higher antibody titer without showing any clinical signs when compared with control animals.

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Kafrelsheikh Veterinary Medical Journal
Volume 9, Issue 1
April 2011
Pages 211-228

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