SOME BIOCHEMICAL AND IMMUNOLOGICAL STUDIES OF PROBIOTICS (BIONUTRA&DINAFERM) IN BROILERS

Document Type : Original Article

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Animal Health Research Institute, provisional lab Kafer El-Sheikh Pharmacology

Abstract

The aim of the present work was to study some biochemical and immunological effects of two probiotics namely Bio nutra and Dinaferm on Hubbard broiler chickens. Birds were divided into three equal groups, each of 50 chicks. The 1st .group received only basal ration (control). The 2nd group received bionutra(1kg/ton) and the 3rd group received dinaferm (1kg/ton). All groups were routinely vaccinated against Newcastle and  were challenged with a virulent strain of ND at the 35th day of age.  The number of dead birds were recorded daily till the end of the experiment.  Birds were sacrificed at 45 days and  blood samples were collected from chickens. Moreover relative weights of bursa, spleen  and thymus were also recorded. It was observed that administration of probiotics has no significant effects on the levels of serum AST, ALT, createnine and uric acid. Moreover, there was insignificant changes in the basophils ,eosinophils ,heterophils ,monocytes and lymphocytes percentagein treated groups.  On the other hand, probiotics induced a significant increase of the number of RBCS and WBCS. Total serum protein  and HI titer increased also in treated groups. Additionally,  Bio- nutra increased the relative weights of bursa and thymus while Dinaferm increased the relative weight of bursa only

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