PREPARATION AND EVALUATION OF DIAGNOSTIC KITS OF NEWCASTLE DISEASE: INFECTIOUS BRONCHITIS AND INFECTIOUS BURSAL DISEASE

Document Type : Original Article

Authors

1 Department of Virology, Faculty of Veterinary Medicine, Kafrelsheikh Univer

2 Department of Virology, Faculty of Veterinary Medicine, Benha University

3 Department of Anatomy, Faculty of Veterinary Medicine, Kafrelsheikh University

4 Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo

Abstract

The present study was designed to prepare ND, IB and IBD anti sera
conjugated with fluorescine isothiocyanate and horse radish
peroxidase to be available on request as local products saving time
and cost. The obtained results indicated that the prepared Newcastle
Disease (NDV); Infectious Bronchitis (IBV) and Infectious Bursal
Disease (IBDV) antisera either in simple forms or conjugated forms
with fluorescine isothiocyanate and horse radish peroxidase are of
high quality providing local kits of low cost and on time of request for
diagnostic and confirmatory serological tests.
In addition, preparation of antisera in goats,which need less husbandry
requirements than those required for chicken or rabbits, provide huge
amounts of such kits in comparison with those prepared in other hosts,
the thing which helps to decrease the cost.
 

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