SOME BIOCHEMICAL AND HAEMATOLOGICAL STUDIES IN BRUCELLOSIS INFECTED BUFFALOES, COWS AND SHEEP IN EL-SHARKIA GOVERNORATE

Document Type : Original Article

Authors

1 Biochemistry Department, Animal Health Research Institute, Dokki

2 Brucella Department, Animal Health Research Institute, Dokki

Abstract

Blood samples from 351 buffaloes, 149 cows and 551 sheep were taken
from a farm at New Salhia, (El-Sharkia Governorate) and examined
for the incidence of brucellosis by the following serological tests,
qualitative buffered acidified plate antigen (BAPAT), Rose Bengal
Plate test (RBPT), the standard quantitative tube agglutination test
(SAT) and the confirmatory quantitative complement fixation test
(CFT). Biochemical and haematological studies were done on 60
seropositive (20 from each species) as well as 60 seronegtaive
animals (20 from each species) as control ones
The bioechmiacl analysis revealed a significant increased enzymatic
activities for both, asparatate aminotransferase (AST) and alanine
aminotransferase (ALT), and a significant increase in urea level of
infected animals as compared with the control ones. On the other
hand, a significant decrease was recorded in total serum protein and
glucose levels in all infected species. Serum iron level showed a
significant decrease in infected cows only. Whereas, no changes were
detected for both serum calcium and inorganic phosphorus levels in
infected animals comparing with control ones.
Concerning haematological profile, we recorded a significant decrease
in Hb and PCV% in all infected species accompanied with a general
decrease in RBCs count, only of significant value in infected cows. The
WBCs count showed insignificant alterations in all infected animals
compared with control ones.
 

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