PHENOTYPIC AND GENOTYPING CHARACTERIZATION OF PASTEURELLA MULTOCIDA IN FARM ANIMALS

Document Type : Original Article

Authors

1 Animal health research institute, Tanta, Egypt.

2 Department of Bacteriology, Mycology & Immunology, Faculty of Veterinary Medicine, Alexandria University.

3 Department of Bacteriology, Mycology & Immunology, Faculty of Veterinary Medicine, Kafrelsheikh University.

4 Bacteriology Research Department, Animal Health Research Institute, Dokki, Egypt

Abstract

Pasteurellamultocida  which  is  the  common  cause  of  respiratory  disease  in  cattle  causing  higheconomic losses. To achieve this objective,  this study succeeded in isolating  a total of27 of P. multocida isolates from 280 samples (9.6%). Lung tissues showed the highestpercentage  of  P.  multocida  isolation  (13.1%),  followed  by  Nasal Swabs  (5%). These isolates were confirmed  microscopically  to  be  P. multocida  bystaining with Gram's  and  Leishman's  stains,  then  biochemically by traditional test likeindole,  catalase,  oxidase,  urease,  citrate  utilization,  sugar  fermentation  and  gelatinliquefaction tests. Also, the results were confirmed using commercial test API 20E andPCR.  For  common gene and capsular serotyping  of  P. multocida  by using multiplex capsular PCR typing system,  a  total  of 9 representative isolates  weretested and 5  isolates were positive for common gene of P. multocida and positive for capsular type E. Positive P. multocida isolates were tested for presence of virulence factors by multiplex PCR for virulence genotyping which represented by virulence genes (TbpA, nanH, ompH and ptfA) which revealed 100% percentages of Tbpa and nanH in lung tissues and nasal swabs in cattle and buffaloes isolates, the presence of ptfA gene in high percentages in lung tissues in buffalo more than in nasal swabs.

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