HYGIENIC STATUS OF INFANT MILK SOLD AT THE MARKETS

Document Type : Original Article

Authors

1 Food control dep. Fac. of Veterinary Medicine Kafrelsheikh University, Egypt.

2 Food hygiene dep. Animal Health Research Institute, Kafrelsheikh branch, Egypt.

Abstract

One hundred of infant milk powder samples were collected from different pharmacies in Kafrelsheikh Governorate for bacteriological examination. The obtained results revealed that 19% of examined samples contained B. cereus in average count of 1.2x102± 5.2x10 cfu/g while 11% of the samples had B. cereus (sporulated form)with an average count of 4.0x102± 1.4x102 cfu/g . 30 strains isolated were screened by PCR technique for hblCgene using FHBLC (F) and FHBLC (R) primers and cytK gene using FCytK (F) and FR2ytK (R) primers. Eight (42%) of vegetative B. cereus isolates had hblC gene and 3 isolates had cytKgene and 7 isolates had both genes. Of the eleven B. cereus spore strains, 4 isolates had hblC gene, 2 isolates had cytKgene and 2 isolates had both genes. E. sakazakii could be isolated from 3% of the examined samples while salmonellae failed to be detected in any of the examined samples. 4 strains , one carrying hblCgene, one carrying cytK gene, one carrying the both genes and one do not carry any of the genes were inoculated into reconstituted milk powder at concentration raged from 5x10 to 1.6x102 cfu/g reconstituted milk. The inoculated milk samples were incubated at 25°C and examined for B. cereus count each 2 hours until 6 hours storage.There was a remarkable increase of B.cereusorganisms count without significance difference between the B.cereus inoculated genes.
The results allow concluding that infant milk powder in spite of its low moisture content may at times be responsible for food poisoning to infants. The public health importance of the isolated microorganisms was discussed.

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