MOLECULAR CHARACTERIZATION OF NEWCASTLE DISEASE VIRUS ISOLATED FROM SAUDI ARABIA

Ismail, Mahmoud M.; Alankari, Abdulrahman; Mohamed, Mahmoud  H. A.; Elkhayat, Faris

doi:10.21608/kvmj.2016.108855

MOLECULAR CHARACTERIZATION OF NEWCASTLE DISEASE VIRUS ISOLATED FROM SAUDI ARABIA

Document Type : Original Article

Authors

¹ Dept. of Poultry Diseases, college of Veterinary Medicine, Kafrelsheikh University, Egypt. Dept. of Clinical Studies, College of Veterinary Medicine and Animal Resources, King Faisal University, Al-Hasa, Saudi Arabia

² Dept. of Clinical Studies, College of Veterinary Medicine and Animal Resources, King Faisal University, Al-Hasa, Saudi Arabia

³ Dept. of Poultry Diseases, college of Veterinary Medicine, Kafrelsheikh University, Egypt

10.21608/kvmj.2016.108855

Abstract

A total of 34 samples were collected from chicken and pigeon flocks suspected to have Newcastle disease. NDV virus was isolated and identified by inoculation into the allantoic cavity of Specific–pathogen- free chicken eggs followed by hemagglutination and hemagglutination inhibition test. Furthermore, these isolates were geno-typically analysed using RT-PCR with specific primers flanking a 270 bp region representing the hypervariable region of F gene and the PCR-product was sequenced and compared to the previously published sequences. In this study, the F protein of 34 Saudi NDV isolates phylogram revealed clustering with NDVs representing lineage 2,4 and 5.

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