DETECTION OF VIRULENCE ASSOCIATED GENES IN LISTERIA MONOCYTOGENES ISOLATED FROM DISEASED FARM ANIMALS

Document Type : Original Article

Authors

1 Pathology Dept. Faculty of Veterinary Medicine Kafrelsheikh University, Egypt.

2 Bacteriology, Mycology and Immunology Dept. Fac. of Vet. Med. Kafrelsheikh Univ., Egypt.

3 Animal health research institute, Mansoura Provincial Laboratory.

4 Genome Research Unite, AHRI

Abstract

A total of 631 different samples (milk, faeces, brain andCerebrospinal fluid) were collected from 268 diseased farm animals and emergency slaughtered animals (80 sheep, 60goat, 75 cattle and 53 buffaloes) from Dakahlia Governoratein 2014 and 2015. The samples were tested for presence of L. monocytogenes by isolation and biochemical identification.
 The results revealed that L. monocytogenes was presented in diseased and emergency slaughtered sheep, goat, cattle and buffaloes in ratio of (36.5%), (28.67%), (24.48%), (17.4%) respectively. Using multiplex PCR technique, detected the presence of virulence-associated genes (inlA, inlC, inlJ, inlB, plcB, hylA, jap and plcA).

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