ISOLATION AND IDENTIFICATION OF CANINE ADENOVIRUS TYPE 2 FROM THE EGYPTIAN DOGS

Kasem, S. G.; Kotb, A. M.; Al-Maria, N. F

doi:10.21608/kvmj.2015.109849

ISOLATION AND IDENTIFICATION OF CANINE ADENOVIRUS TYPE 2 FROM THE EGYPTIAN DOGS

Document Type : Original Article

Authors

¹ Department of Virology, Faculty of Veterinary Medicine, Kafrelsheikh University

² Veterinary Serum and Vaccine Research Institute. Abbassia, Cairo, Egypt

³ Dept. of Agric. Botany, EPCRS Excellence Center, Fac. of Agric. Kafrelsheikh

10.21608/kvmj.2015.109849

Abstract

Canine infectious tracheobronchitis (kennel cough) caused by CAV-2 is one of the most prevalent respiratory infections of dogs. Kennel cough characterized by frequent dry and hacking coughing high morbidity but low mortality rate. In this study, nasal and ocular discharge samples have been taken from 59 dogs from the veterinary clinics of pet animals in Cairo, Egypt of different age and sex from 2013 to 2014. It showed clinical symptoms, a systemic disease which characterized by fever, diarrhea, vomiting, oculonasal discharge, conjunctivitis, severe moist cough, signs of pulmonary disease and dehydration. Isolated virus was identified by qualitative chromatographic immunoassay by using Anigen Rapid CIRD-3 Ag Test Kit, the result was positive for CAV in only 7 samples. Then the virus from positive samples was passaged for 3 blind passages in Vero cell then subjected to FAT and haemagglutination tests, the results indicated that the virus was most likely to be present in the respiratory tract. The virus was processed and inoculated onto confluent monolayers of MDCK, Vero, BHK21, and MDBK cells for 10 passages using standard virological techniques. The Cytopathic effect (CPE) appeared and MDCK cells were the most susceptible cell culture where they gave the highest infectivity titre.

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