OCCURRENCE OF QUINOLONE RESISTANCE GENES AMONG SALMONELLA SPECIES ISOLATED FROM CHICKENS

Document Type : Original Article

Authors

1 Bacteriology, Mycology and Immunology Department, Faculty of Veterinary Medicine, Kafrelsheikh University

2 Animal Health Research Institute, Dakahlia branch

3 Bacteriology, Mycology and Immunology Department, Faculty of Veterinary Medicine, Kafrelsheikh University.

4 Animal Health Research Institute, Dokki Lab., Giza.

Abstract

Resistance to antimicrobial agents within nontyphoidal Salmonella serotypes is considered a serious problem worldwide so this study was aimed to investigate the wide spread Plasmid-mediated quinolone resistance between different Salmonellae isolated from chickens .The antimicrobial susceptibility was applied on all isolates then PCR was applied for most resistant isolates to quinolones to detect the Plasmid-mediated quinolone resistance genes (qnrA, qnrB, qnrS). Forty one Salmonella isolates (9.1%) were obtained from 450 samples. All isolates exhibit resistance against all antimicrobial agents except amikacin in which give no resistance. All isolates showed resistance with 100% against nalidixic acid and flumequin but 4 isolates showed the lowest resistance (9.7%) to levofloxacin. Five isolates were sensitive to amoxicillin giving the lowest percentage (12.1%). Twenty two Salmonella isolates from a total of 41 isolates were examined by PCR for Plasmid-mediated quinolone resistance genes (qnrA, qnrB, and qnrS). while 19 Salmonella isolates not examined as they were less resistant to quinolones. Five isolates (5/22) (22.7%) were positive for qnrA, 2 isolates (2/22) (9%) were positive for qnrB and 4 isolates (4/22) (18%) were positive for qnrS.

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Kafrelsheikh Veterinary Medical Journal
Volume 13, Issue 2
October 2015
Pages 129-148

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