MOLECULAR CHARACTERIZATION OF SOME GRAM-NEGATIVE BACTERIA ISOLATED FROM MILK

Document Type : Original Article

Authors

1 Department of Bacteriology, mycology and immunology, Faculty of Veterinary Medicine, Kafrelsheikh University

2 Faculty of Aquatic Wealth and Fisheries, Kafrelsheikh University

Abstract

Milk isn’t only a perfect food, but also an ideal media for microbes, thus it is a major pathogen vehicle. This study was conducted to characterize molecularly some of Gram-negative pathogens in random150 raw milk samples (100 from healthy dairy farm animals and 50 from milk vendors) collected from ElmahallaElkobra and Mansoura cities, Egypt, then 20 farm samples with subclinical mastitis were excluded. Gram-negative bacteria were isolated and identified biochemically from the other 130 samples; the predominant species percentages were Escherichia coli 25.4, Serratiamarcescens 13.8, Kluyveraascorbata 8.5, Citrobacterdiversus 7.7, Klebsiellaoxytoca 5.4, and Klebsiellapneumoniae3.8. 18 shigatoxin producingE. coli (STEC) strains were classified serologically to 8 non-O157 serotypes, and the predominant was O26:H11 (27.78%). By PCR;16S-23S rDNA was identified in 3 K. pneumoniae, and shiga toxins (stx1 and 2), α-haemolysin (hlyA) and intimin (eaeA) genes were detected in 15, 13, 7 and 9 STEC respectively. Conclusively, raw milk still contributes a public hazard,so, better effective control measures are required.

Keywords

Main Subjects

Files

Share

How to cite

Statistics