Document Type : Original Article
Authors
1
Animal Health Researches Institute, Aswan branch, Egypt
2
Department of Animal Medicine, faculty of Veterinary Medicine, South Valley University, Qena city, Qena 83523, Egypt.
3
Animal Health Researches Institute, Dokki, Giza, Egypt
Abstract
A total of 150 raw milk samples were collected from she camels from Aswan, 100 from individual breed in Daraw and 50 from pastoral camels in Shalateen in Egypt. First, clinical examination revealed no cases with clinical mastitis. Second, the collected milk samples were tested by California Mastitis Test and the results revealed that the percent of subclinical mastitis was (39.3%) for total number of milk samples, but the ratio in Daraw (48%) higher than in Shalateen (22%). Third, by conventional culture method, 20 % of samples were positive to Staphylococcus spp. Fourth, Staphylococcal isolates were identified by conventional biochemical test, (46.7%) of these isolate showed positive for coagulase test. Fifth, these coagulase positive isolates submitted for molecular identification targeting 16S rRNA gene (Staphylococcus genus specific), nuc gene (S. aureus species specific) and Staphylococcal enterotoxins genes (SEA, SEB andSEC) by multiplex PCR, (92.9%) positive for 16s rRNA and confirmed as Staphylococcus spp., (64.3%) positive for nuc gene and confirmed to be S.aureus and (50%) Staphylococcus isolates were enterotoxin-positive to sea, (35.7%) of them for S.aureus strains and (14.3%) for other coagulase positive Staphylococcus spp. Finally, these isolates were submitted for antibiotic sensitivity testing by using various antibiotics and the proportion of isolates resistance to the antibiotics were penicillin G and chloramphenicol (100%), colistin (93%), tetracycline and lincomycin (78.6%), oxalinic acid (71.4%), amoxicillin (50%), neomycin and cefaclor (42.8%), erythromycin (35.7%), ciprofloxacin (28.6%) and gentamycin (21.4%).
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