MOLECULAR SURVEY OF AVIAN RESPIRATORY VIRUSES IN COMMERCIAL BROILER AND LAYER FLOCKS WITH RESPIRATORY MANIFESTATIONS IN KAFRELSHEIKH GOVERNORATE

Document Type : Original Article

Authors

1 Departement of Poultry Diseases, Faculty of Veterinary Medicine, Kafrelsheikh University

2 Departement of Virology, Faculty of Veterinary Medicine, Kafrelsheikh University

3 Departement of Virology, Faculty of Veterinary Medicine, Banha University

Abstract

Acute respiratory tract infections are generally the main hazard to the poultry industry in Egypt leading to severe economic losses. The present study was aimed to standardize a multiplex polymerase chain (mPCR) reaction that simultaneously detects and differentiates the four major avian respiratory viruses (AIV, NDV, IBV and ILTV) in single reaction, as well as to apply a molecular survey of avian respiratory viruses in commercial broiler and layer flocks suffering from respiratory manifestations with relatively high mortality rate in different localities of Kafrelsheikh governorate using the standardized mPCR assay. The obtained results indicate the high occurrence of mixed infections among these viruses which makes it difficult to differentially diagnose these diseases by clinical manifestations.

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