QUANITATIVE ANALYSIS OF SOME BIOGENIC AMINES IN SALTED FISH IN RELATION TO THEIR NACL CONCENTRATIONS

Document Type : Original Article

Authors

1 Animal Health Research institute, Tanta branch.

2 Food Control Department, Faculty of Veterinary Medicine, Kafrelshiekh University

Abstract

The main objective of this study is to analyze the salted fish to determine the concentrations of biogenic amines in relation to their sodium chloride concentrations. Forty random samples of salted Sardine and Mugil cephalus fish” Feseekh” (20 of each)  were collected from different fish markets in El- Gharbia and Kafr El-shiekh Governorates, Egypt. The collected samples were packed in polyethylene bags and stored at -20ºC till be analyzed for determination of  their sodium chloride concentrations by titration method and biogenic amines using High Performance Liquid Chromatography(HPLC). The obtained results revealed that the mean sodium chloride concentrations were 12.72 ± 4.02 and 14.20 ± 3.18 for salted Sardine and Feseekh, respectively. On the other hand the mean concentration of histamine was 19.26± 11.83 and 16.02±10.54 mg/ 100g of the examined salted sardine and feseekh samples, respectively .While the mean concentration of tyramine was  13.03± 8.55 and 11.21± 7.28, respectively for the same examined samples. Concerning to cadeverine the mean concentration was 10.09± 6.04 and 7.84± 5.64, respectively. Comparing the obtained results with the permissible limit (20mg/100g) recommended by FDA (2001), 35, 20 and 10% of  the examined salted Sardine samples exceeded the limit for histamine, tyramine and cadaverine, while 30, 20 and 5% of the examined feseekh samples exceeded the permissible  limit for histamine, tyramine and cadaverine, respectively. This study show that there is a Significant negative correlation between sodium chloride percent  and biogenic amines concentration  in  the examined salted fish samples for histamine While there is unsignificant negative correlation between sodium chloride percent and cadaverine concentration in the examined salted fish samples

Keywords

Main Subjects