TAURINE AND CAFFEINE-CONTAINING ENERGY DRINKS: LUMINAL PRESENCE OF TAURINE REDUCES BACLOFEN INTESTINAL ABSORPTION IN RATS

Leisen, C.; Hanafy, A.; Spahn-Langguth, H.

doi:10.21608/kvmj.2004.112435

TAURINE AND CAFFEINE-CONTAINING ENERGY DRINKS: LUMINAL PRESENCE OF TAURINE REDUCES BACLOFEN INTESTINAL ABSORPTION IN RATS

Document Type : Original Article

Authors

¹ Department of Pharmaceutical Chemistry, School of Pharmacy, Martin-Luther-University Halle-Wittenberg, Germany

² Department of Pharmacology and Forensic Medicine, Faculty of Vet. Med., Kafr El-Sheikh, Tanta University, Egypt

10.21608/kvmj.2004.112435

Abstract

The absorption of baclofen (BAC), a centrally acting antispastic agent with γ-amino acid structure as well as the influence of taurine (TAU) and caffeine (CAFF) on baclofen permeability was investigated by in-situ single-pass rat intestinal perfusion. Taurine is known to be transp-ortedbyβ-aminoacidcarrier,atransporterthat ispresumed to be shared by baclofen in its intestinal absorption. The aim of this study was to characterize the intestinal transport properties of baclofen in three different intestinal segments and to demonstrate the influence of co-administration of taurine as a competitive inhibitor of active baclofen absorption. Jejunum, ileum and colon of male White Wistar rats (n=4) were perfused simultaneously. Baclofen concentration was 0.1 mM, a concentration,atwhichbaclofentransportisnot yet saturated.Following 30 minutes perfusion with pure baclofen in tyrode solution, 400mg/dl taurine (32 mmol/l), resp. 30 mg/dl caffeine (0.62 mmol/l) were added solely as well as in combination to the perfusion solution and the perf-usion was continued in the same animal up to 90 minutes. Taurine and caffeine concentrations corresponded to those usually used in energy drinks. Additionally, baclofen permeability was directly studied using a typical energy drink (ED)Red Bull® instead of tyode.The perfusates were collected and baclofen concentrations were quantified by HPLC. The intestinal permeabilities (Peff) were determined on the basis of the differences between the inlet and outlet concentrations. Results from in-situ intestinal perfusions in jejunum showed that Peff values are approximately reduced to 19.5% following addition of taurine, 10.4% by addition of taurine plus caffeine. The energy drink reduced baclofen permeability to 13.9%. The effect of taurine and caffeine in ileum and colon was similar to those found in jejunum.

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