Document Type : Original Article
Authors
1
Dept. of Bacteriology, Mycology and Immunology, Fac. Vet. Med., Kafrelsheikh Univ., Egypt
2
Dept. of Microbiology, Animal Health Research institute, Dokki.,Giza, Egypt.
Abstract
Out of 308 samples 205 from apparently healthy and 103 diseased lung of slaughtered camels. The total number of positive samples was 203 (65.91%) from which 124 (60.49%) from apparently healthy and 79 (76.70%) from pneumonic lung of camels.
The total number of isolates was 302 isolates from which 174 isolates from apparently healthy and 128 isolates from pneumonic lung
of camels. The total number of Gram negative isolates 170 (56.29%) from which isolates were 94 (54.02%) and 76 (59.38%) in apparently healthy and diseased lungs of camel respectively as shown in
table (3).
On the other hand the main predominant single Gram negative isolates were E. coli 28 samples (26.92%), Pseudomonas aeruginosa 9 samples (8.65%), Citrobacter freundii 7 samples (6.73%) , Klebsiella pneumoniae 6 samples (5.77%), Citrobacter spp. 5samples (4.81%), Klebsiella oxytoca 3 samples (2.89%), Mannheimia haemolytica and Yersinia enterocolitica 2 samples (1.92%), Klebsiella ozaenae and Pasteurella multocida one sample (0.96%).
Serological identification from apparently and pneumonic lung of slaughtered camels was 44 isolates of E. coli including 29 isolates from apparently healthy as E. coli O111, E. coli O115, E.coli O119 E. coli O146 and E. coli O166 and 20 isolates from pneumonic lung including E. coli O55, E. coli O111 E. coli O115, E. coli O119, E. coli O125, E. coli O146 and E. coli O166.
Regarding to the pathogenicity of gram negative isolates in experimentally infected mice by 0.1 ml (5 x 108 cFm. ml). It was fatal to mice with varying pathogenicity, Klebsiella pneumoniae, E. coli O55, E. coli O115, Pseudomonas aerouginosa, Pasteurella multocida and Mannheimia haemolytica were 100%. Meanwhile the lowest pathogenic bacteria were E. coli O119 and Proteus vulgaris were 20%. But citrobacter spp. and Proteus penneri not causing deaths among mice.
Chloramphenicol, Ciprofloxacin, Enrofloxacin, Flumequine, Gentamicin and Norfloxacin against Citrobacter freundii, Citrobacter spp., E. coli, Enterobacter cloacae, K. oxytoca, K. ozaenae and K. pneumoniae. Ciprofloxacin, Doxycycline, Enrofloxacin and Norfloxacin against Mannheimia haemolytica, Pasteurella multocida, Proteus penneri, Proteus vulgaris, Ps. aeruginosa and Yersinia enterocolitica.
SDS-PAGE analysis of Klebsiellae and E. coli showed that they contained from 5 to 12 bands after staining with Commiassie brilliant blue method which molecular weight ranged from 7.218 to 170.97 KDa.
It was obvious that the molecular weight of E. coli O55 ranged from 134.55 to 7.2118 KDa. The molecular weight of E. coli 111 ranged from 134.55 to 32.208 KDa. The molecular weight of E. coli O115 ranged from 134.55 to 33.311 KDa. The molecular weight of E. coli O119 ranged from 131.24 to 8.4471 KDa. The molecular weight of E. coli O125 ranged from 118 to 28.562 KDa. The molecular weight of E. coli O146 ranged from 151.11 to 11.741 KDa. The molecular weight of E. coli O166 ranged from 154.41 to 21.137.
On the other hand the molecular weight of Klebsiella oxytoca ranged from 157.72 to 15.859 KDa. The molecular weight of Klebsiella pneumoniae ranged from 167.66 to 22.15 KDa. The molecular weight of Klebsiella ozaenae ranged from 170.97 to 38.016 KDa.
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