USE OF POLYMERASE CHAIN REACTION FOR DIAGNOSIS OF BOVINE CORONAVIRUS IN DIARRHEIC CALVES

Document Type : Original Article

Authors

1 Animal Health Research Institute, Dokki, Giza

2 Animal Reproduction Research Institute, Al-Harram, Giza

3 Animal Health Research Institute, Dokki, Giza.

Abstract

Bovine Corona virus (BCV) is the main cause of diarrhea in many animal species world wide. Calves up to 3 months old can be affected by this virus. The importance of BCV in cattle industry is due to economic loss, treatment costs, and reduced growth rate in beef and dairy calves. This study dimed to examine 68 fecal samples(50 from diarrheic  and 18 from non diarrheic calves) by using reversed transcriptase-polymerase chain reaction (RT-PCR), antigen capture  enzymes linked Immunosorbent Assay (ELISA),direct Flourescent antibody test (FAT) and Haemagglutination and Haemagglutination inhibition technique  (HAT/HIT) for detecting of BCV in diarrheic and non diarrheic calves. The highest percentage of BCV in diarrheic calves by RT-PCR was 84 % then 76% by using BCV antigen capture ELISA then 62% by direct FAT and the lowest percentages was 56% by HIT. Also the apparent prevalence of BCV in non diarrheic calves were 44.4% by RT-PCR 27.7 by antigen capture ELISA, 22.2% by direct FAT and 11.1% by HIT respectively. These results suggested that RT-PCR is more sensitive   than other diagnostic methods to detect BCV, especially in subclinical cases.

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Kafrelsheikh Veterinary Medical Journal
Volume 9, Issue 2
October 2011
Pages 149-164

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